TYROSYL-TRNA SYNTHETASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1h3e(A) : *HTPEEALALLKRGAEEIVPEEELLAKLKEGRPLTVKLGADPTRPDLHLG           :                                                      1h3f(B) : GHTPEEALALLKRGAEEIVPEEELLAKLKEGRPLTVKLGADPTRPDLHLG             __________________________________________________             __________________________________________________   1h3e(A) : HAVVLRKMRQFQELGHKVVLIIGDFTGMIGDPSGRSKTRPPLTLEETREN           :                                                      1h3f(B) : HAVVLRKMRQFQELGHKVVLIIGDFTGMI-------------tLEETREN             __________________________________________________             __________________________________________________   1h3e(A) : AKTYVAQAGKILRQEPHLFELRYNSEWLEGLTFKEVVRLTSLMTVAQMLE           :                                                      1h3f(B) : AKTYVAQAGKILRQEPHLFELRYNSEWLEGLTFKEVVRLTSLMTVAQMLE             __________________________________________________             __________________________________________________   1h3e(A) : REDFKKRYEAGIPISLHELLYPFAQAYDSVAIRADVEMGGTDQRFNLLVG           :                                                      1h3f(B) : REDFKKRYEAGIPISLHELLYPFAQAYDSVAIRADVEMGGTDQRFNLLVG             __________________________________________________             __________________________________________________   1h3e(A) : REVQRAYGQSPQVCFLMPLLVGLDGREKMSKSLDNYIGLTEPPEAMFKKL           :                                                      1h3f(B) : REVQRAYGQSPQVCFLMPLLVGLDGREKMSKSLDNYIGLTEPPEAMFKKL             __________________________________________________             __________________________________________________   1h3e(A) : MRVPDPLLPSYFRLLTDLEEEEIEALLKAGPVPAHRVLARLLTAAYALPQ           :                                                      1h3f(B) : MRVPDPLLPSYFRLLTDLEEEEIEALLKAGPVPAHRVLARLLTAAYALPQ             __________________________________________________             ___________________________________342____________   1h3e(A) : IPPRIDRAFYESLGYAWEAFGRDKEAGPEEVRRAEARYDEVAKGGIPEEI           :                                                      1h3f(B) : IPPRIDRAFYESLGYAWEAFGRDKEAGPEEVRRAEARYDe---------I             ___________________________________342____________             __________________________________________________   1h3e(A) : PEVTIPASELKEGRIWVARLFTLAGLTPSNAEARRLIQNRGLRLDGEVLT           :                                                      1h3f(B) : PEVTIPASELKEGRIWVARLFTLAGLTPSNAEARRLIQNRGLRLDGEVLT             __________________________________________________             ____________________________                         1h3e(A) : DPMLQVDLSRPRILQRGKDRFVRVRLSD                                 :                                                      1h3f(B) : DPMLQVDLSRPRILQRGKDRFVRVRLSD                                   ____________________________

Details

Property   Value  Conformer 1 1h3e(A)  Conformer 2 1h3f(B)  Radius gyration for conformer 1 25.33 A  Radius gyration for conformer 2 29.97 A  EC Number 6.1.1.1   Click here to see the DynDom results or the famliy  Trigger ligands ATP1433 TYB1434  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands No  Residues in enzyme contacting ligands ALA44  ARG93  ASN128  ASP182  ASP196  ASP45  ASP85  GLN179  GLN197  GLU191  GLY193  GLY194  GLY43  GLY54  HIS52  HIS55  ILE75  LEU223  LEU224  LYS232  LYS235  LYS41  MET233  PRO222  SER234  TYR108  TYR175  VAL57  VAL58   Residues in extended bending regions contacting ligands Null  Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT