UDP-N-ACETYLMURAMATE-L-ALANINE LIGASE
Ligand-induced domain movement details

Jmol view of liganded conformation 1

Domain Movement

domain 1 is binding domain(fixed in space)	domain 2 is binding domain(fixed in space)

Sequence

__________________________________________________   1p3d(A) : IIPErRVQQIHFIGIGGAG-sGIAEILLNEGYQISGSDIADGVVTQRLAQ           :                                                      1gqq(B) : *******QQIHFIGIGGAGMSGIAEILLNEGYQISGSDIADGVVTQRLAQ             __________________________________________________             _____________________________________________109__   1p3d(A) : AGAKIYIGHAEEHIEGASVVVVSSAIKDDNPELVTSKQKRIPVIQRAQ-l           :                                                      1gqq(B) : AGAKIYIGHAEEHIEGASVVVVSSAIKDDNPELVTSKQKRIPVIQRAQML             _____________________________________________109__             __________________________________________________   1p3d(A) : AEI-rFRHGIAVAGTHGKTTTTAi--SiYTQAKLDPTFVNGGLVKSAGKN           :                                                      1gqq(B) : AEIMRFRHGIAVAGTHGKTTTTAMISMIYTQAKLDPTFV-----------             __________________________________________________             __________________________________________________   1p3d(A) : AHLGASRYLIAEADESDASFLHLQP-vSVVTN-ePDHdTYEGDFEK-kAT           :                                                      1gqq(B) : ------rYLIAEADE----fLHLQPMVSVVTNMEP--------fEKMKAT             __________________________________________________             __________________________________________________   1p3d(A) : YVKFLHNLPFYGLAV-cADDPVL-eLVPKVGRQVITYGFSEQADYRIEDY           :                                                      1gqq(B) : YVKFLHNLPFYGLAVMCADDPVLMELVPKVGRQVITYGFSEQADYRIEDY             __________________________________________________             __________________________________________________   1p3d(A) : EQTGFQGHYTVICPNNERINVLLNVPGKHNALNATAALAVAKEEGIANEA           :                                                      1gqq(B) : EQTGFQGHYTVICPNNERINVLLNVPGKHNALNATAALAVAKEEGIANEA             __________________________________________________             __________________________________________________   1p3d(A) : ILEALADFQGAGRRFDQLGEFIRPNGKVRLVDDYGHHPTEVGVTIKAARE           :                                                      1gqq(B) : ILEALADFQGAGRRFDQLGEFIRPNGKVRLVDDYGHHPTEVGVTIKAARE             __________________________________________________             __________________________________________________   1p3d(A) : GWGDKRIV-iFQPHRYSRTRDLFDDFVQVLSQVDALI-lDVYAAGEAPIV           :                                                      1gqq(B) : GWGDKRIVMIFQPHRYSRTRDLFDDFVQVLSQVDALIMLDVYAAGEAPIV             __________________________________________________             __________________________________________________   1p3d(A) : GADSKSLCRSIRNLGKVDPILVSDTSQLGDVLDQIIQDGDLILAQGAGSV           :                                                      1gqq(B) : GADSKSLCRSIRNLGKVDPILVSDTSQLGDVLDQIIQDGDLILAQGAGSV             __________________________________________________             _____________                                        1p3d(A) : SKISRGLAESW**                                                :                                                      1gqq(B) : SKISRGLAESWKN                                                  _____________

Details

Property   Value  Conformer 1 1p3d(A)  Conformer 2 1gqq(B)  Radius gyration for conformer 1 22.52 A  Radius gyration for conformer 2 24.05 A  EC Number 6.3.2.8   Click here to see the DynDom results or the famliy  Trigger ligands UMA602  Trigger ligands in which conformation Conformation  1  Conformaton with trigger ligands is compact Yes  Spanning ligands Yes  Residues in enzyme contacting ligands ALA29  ALA459  ALA86  ARG107  ARG377  ARG380  ASP175  ASP49  GLU173  GLY152  GLY25  GLY27  GLY28  GLY30  HIS198  HIS348  HIS376  HIS70  ILE50  ILE87  SER177  SER84  SER85  TYR346   Residues in extended bending regions contacting ligands ARG107   Residues in extended bending regions making H-bonds or salt-bridges with ligands Null   Residues in extended bending regions with an H-bond between its main chain and ligands Null Click here to see the details of H-bonds between ligands and enzyme in PDF format generated by LIGPLOT